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a Mean fluorescence intensity (MFI) of CD28 in CD4+CD28+ subpopulation in IC and PB samples. The MFI of CD28 in CD4+CD28+ subset was significantly lower in IC sample (p = 0.01, Wilcoxon signed rank test). b Representative flow cytometry histogram showing CD28 expression in IC (plain line) and PB (dotted line) samples
