Table 1.

Published genome-wide distributions of Ogt and O-GlcNAcylated proteins

O-GlcNAcylated proteins have been profiled in flies, worms, and mammals using either pan O-GlcNAc antibodies RL2 (described in Holt et al. 1987) or HGAC85 (described in Turner et al. 1990) or using the lectin wheat germ agglutinin (WGA). Examples of ChIP-seq profiles obtained using a monoclonal antibody raised against a synthetic histone H2B peptide GlcNAcylated on serine 112 (Fujiki et al. 2011) are shown in Fig. 2 (see text for details). Direct binding of Ogt itself to chromatin has thus far only been reported in mammalian cells but not in flies or worms