Figure 6.

Heterozygous deletion of BiP in oligodendrocytes exacerbates the course of EAE. A, B, CC1 immunostaining of 8-week-old mice showing no difference in the numbers of mature oligodendrocytes in different regions of the CNS in mice with one allele of BiP (OL-BiP^wt/ko mice) compared with the control (OL-BiP^wt/flox mice). Scale bars, 20 μm. n = 3 animals. C, MBP immunostaining of 8-week-old mice showing normal expression of MBP in different regions of the CNS of OL-BiP^wt/ko mice compared with OL-BiP^wt/flox mice. Scale bars, 20 μm. n = 3 animals. D, OL-BiP^wt/flox mice showed exacerbated EAE disease severity compared with OL-BiP^wt/flox mice. Error bars indicate SED; *p < 0.05; #p < 0.001. In addition, a series of nonparametric Wilcoxon rank-sum tests were performed for each EAE time point and the ANOVA results were confirmed.