Table 2.
List of primary antisera
| Target protein | Host | Dilution | Manufacturer | Catalog no. | RRID | Lot |
|---|---|---|---|---|---|---|
| GFP | Chicken | 1:2000 | Aves | GFP-1010 | AB_2307313 | 0511FP12 |
| GNAT3 | Goat | 1:500 | Aviva System Biology | OAEB00418 | AB_10882823 | G2 E060911 |
| Gustducin | Rabbit | 1:5000 | Santa Cruz | SC-395 | AB_673678 | H3112 |
| P2X3 | Guinea pig | 1:750 | Neuromics | GP10108 | AB_2283325 | 400911 |
| P2X3 | Human | 5 μg/ml | Integral Molecular | n/a | n/a | LM-021 |
| Serotonin | Rabbit | 1:5000 | Immunostar | 20080 | AB_572263 | 924005 |
Both P2X3 antisera were validated by lack of staining of tissue from P2X3-KO mice. LM-021 is a humanized monoclonal antibody directed against the full-length protein using lipoparticle-based immunization (Banik et al., 2011).