Fig. 2.

Time-dependent macrophage infiltration into the adventitia of mesenteric arteries (MAs) but not skeletal muscle arteries of DOCA-salt hypertensive rats. A and B: whole mount immunohistochemical labeling of CD163-positive macrophages in the adventitia of MAs from DOCA-salt (A) and sham control rats (day 21; B). C: normalized number of macrophages per region. Macrophage numbers were four to five times higher in arteries from DOCA-salt rats compared with those from sham control rats beginning on days 10–13. Data are means ± SE and were analyzed by one-way ANOVA and a Bonferonni's post hoc test; n = 5. *P < 0.05 vs. control and days 3–5. D and E: whole mount immunohistochemical labeling of MA (D) and skeletal muscle (E) perivascular sympathetic nerves labeled by neuropeptide Y (NPY) immunoreactivity along with macrophage labeling with anti-CD163. Macrophages were found in close proximity to periarterial sympathetic nerves in MAs but not skeletal muscle arteries from DOCA-salt rats.