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. 2015 Jul 29;309(7):R747–R756. doi: 10.1152/ajpregu.00148.2015

Fig. 2.

Fig. 2.

Knocking down Irk1 in the principal cell of the tubule decreases transcript levels but has no effect on K+ flux. The GAL4-UAS system, using the principal cell GAL4 driver c42-GAL4, was used to knock down Irk genes in the principal cells of the tubule. A: transepithelial K+ flux (pmol/min per tubule) was measured in tubules from control flies (w;c42-GAL4/+ and w/yw;UAS-Irk1RNAi/+) or from flies in which Irk1 was knocked down in the principal cells under the control of c42-GAL4 (w/yw;c42-GAL4/UAS-Irk1RNAi). K+ flux was unchanged in the Irk1 knockdown tubules; n = 13–14 tubules/genotype. P = 0.2537, 1-way ANOVA. B: Irk1 transcript levels were measured using quantitative RT-PCR from control tubules (w;c42-GAL4/+) or from tubules in which Irk1 was knocked down in the principal cells (w/yw;c42-GAL4/UAS-Irk1RNAi). Transcript levels were decreased in the knockdown tubules; n = 4 pairs of 50 tubules/genotype. **P < 0.01.