Table 1.
Genotype | Secretion Rate, nl/min per tubule | [K+], mM | K+ flux, pmol/min per tubule | n |
---|---|---|---|---|
Effect of 2 mM barium | ||||
wBerlin | ||||
Baseline | 0.29 ± 0.048 | 139 ± 7.8 | 41 ± 7.5 | 7 |
After vehicle | 0.37 ± 0.057 | 157 ± 8.3 | 68 ± 16.7 | 7 |
Baseline | 0.31 ± 0.060 | 150 ± 21.9 | 43 ± 9.0 | 8 |
After barium | 0.01 ± 0.010 | 156 ± 19.1 | 9 ± 7.0 | 8 |
GAL4 control: w;c42-GAL4/+ | ||||
Baseline | 0.21 ± 0.028 | 117 ± 6.4 | 25 ± 3.6 | 10 |
After barium | 0.02 ± 0.028 | 116 ± 11.0 | 4 ± 1.6 | 10 |
Irk1 knockdown: w/yw;UAS-Irk1RNAi/c42 | ||||
Baseline | 0.23 ± 0.028 | 116 ± 10.3 | 28 ± 4.6 | 9 |
After barium | 0.05 ± 0.029 | 121 ± 10.2 | 8 ± 3.5 | 9 |
Irk2 knockdown: w;UAS-Irk2RNAi/+;c42-GAL4/+ | ||||
Baseline | 0.24 ± 0.024 | 129 ± 11.0 | 32 ± 4.6 | 11 |
After barium | 0.03 ± 0.010 | 131 ± 10.8 | 6 ± 1.7 | 11 |
Irk1/Irk2 double knockdown: w;UAS-Irk2RNAi/+;c42-GAL4/UAS-Irk1RNAi | ||||
Baseline | 0.18 ± 0.032 | 105 ± 10.6 | 20 ± 4.4 | 10 |
After barium | 0.08 ± 0.022 | 123 ± 12.5 | 13 ± 4.1 | 10 |
All values shown are means ± SE. Note that K+ flux was calculated separately for each tubule analyzed, and therefore the value of mean secretion rate × mean [K+] may differ slightly from measured K+ flux, attributable to rounding.