Fig. 1.
Levels of glutathione peroxidase 1 (GPx1) protein and GPx activity are increased by cardiac ErbB2 overexpression. A and B: whole heart homogenates (n = 4 per group) and mitochondria (n = 8 per group) isolated from the ErbB2tg hearts have higher GPx activity than wild-type (WT) hearts. C: glutathione (GSH) levels are not different between WT and transgenic (TG) animals (n = 6 per group). D: representative immunoblot shows higher expression of GPx1 in whole heart homogenates of transgenic hearts compared with WT hearts (n = 12 per group). E: GPx1 is elevated in cytosolic and mitochondrial fractions from ErbB2tg hearts as shown in representative immunblots (n = 6 per group). Values represent means ± SD. Akt is the loading control protein, and values are fold increase over controls. *P < 0.05.