Fig. 7.

ErbB2 overexpression in H9c2 cells protects against dox-induced oxidative stress and cell death. A: H9c2 cells were transfected with pcDNA (3.1)+ vector or ErbB2 plasmid. B: representative immunoblot shows that transfection with ErbB2 plasmid increases the levels of total and phosphorylated ErbB2 proteins (n = 6 per group). C: after 48 h of transfection the cells were incubated with vehicle or dox for 6 h. ROS production was determined using the DCF-DA fluorescent assay for general oxidative stress (n = 6 per treatment group). D: cell viability was determined by the MTT assay (n = 6 per treatment group). Values represent the means ± SD. GAPDH is the loading control protein and values are fold increase over controls. *P < 0.05; two-way ANOVA < 0.05; *P < 0.05 vs. vector with 0 μM dox; #P < 0.05 vs. ErbB2 with 0 μM dox; †P < 0.05 vs. vector with dox dose.