Fig. 5.

Angiopoietin-1 (Ang-1*) decreases the vascular permeability in the mesenteric vessels of mice treated with rAd-FGF-2 + PPS. FVB/N mice were injected with rAd-FGF-2 alone or in combination with rAd-Ang-1* (5 × 10⁸ pfu/mouse for each adenoviral vector). Seven days later, all mice were injected with PPS (60 mg/kg), anesthetized, and injected retro-orbitally with 100 μl of Evans Blue (EB) dye (5 mg/ml in PBS). The EB injection alone produced a homogeneous bluish staining of the gastrointestinal tract in control mice (B) compared with control mice not injected with EB (A). C: a representative sample of mice treated with PPS plus rAd-FGF-2 demonstrating more significant leakage of EB compared with mice treated with PPS + rAd-FGF-2 + rAd-Ang-1* (D). E: the leakage of the EB dye was quantified in fluid collected from the peritoneal cavity, using arbitrary optical density units, and the results are expressed as means ± SE (n = 3 mice per group; one way ANOVA **P < 0.001 compared with control mice; ##P < 0.001 compared with mice treated with rAd-FGF-2 + PPS). Bars = 0.5 cm.