Table 1.
Coagulation and hematological analysis in blood samples collected from anesthetized mice 7 days after the adenoviral infection, and 1.5 h after the injection of a single dose of PPS (60 mg/kg)
| Group | PT, s | aPTT, s | Bleeding time, s | Platelets count, × 109/liter |
|---|---|---|---|---|
| Control | 9 ± 0.9 | 25 ± 3.3 | 123 ± 21 | 640 ± 197 |
| Control + PPS | 10 ± 0.7 | >300*a | >240*b | 659 ± 146 |
| Ad-Lac-Z | 10 ± 0.5 | 20 ± 2.1 | 143 ± 17 | 740 ± 199 |
| Ad-FGF-2 | 9 ± 0.7 | 19 ± 0.4 | 144 ± 47 | 623 ± 193 |
| Ad-Lac-Z + PPS | 12 ± 2.1 | >300*a | >240*b | 764 ± 87 |
| Ad-FGF-2 + PPS | 10 ± 1.1 | >300*a | >240*b | 648 ± 138 |
| Ad-FGF-2 + Ad-Ang-1* + PPS | 11 ± 1.2 | >300*a | >240*b | 647 ± 163 |
All values are expressed as the means ± SD.
Prothrombin time (PT) and activated partial thromboplastin time (aPTT) were measured in blood collected through a heart puncture (5-14 mice per group). Platelet counts and bleeding times were measured in blood collected from the tail as described in methods. PPS, pentosan polysulfate; FGF-2, fibroblast growth factor-2; rAd, recombinant adenovirus; ND, not done.
aPTT > 300 s cannot be accurately analyzed with our assay.
Tail bleeding times were terminated after 240 s.
Kruskal-Wallis test P value = <0.0001 (n = 5–14 per group).