Postnatal maturation of rat arterial smooth muscle gene expression in regional circulations. RNA was isolated from the entire mesenteric arcade (A), femoral artery (B), main renal artery (C), and renal interlobar arteries (D) at the indicated postnatal day (PND). mRNAs were measured by quantitative PCR using Taqman probes, normalized to cyclophilin A, and reported as fold-change vs. PND1. Insets: smooth muscle myosin heavy chain (smMHC) mRNAs at different PNDs. Values are means ± SE with n = 5/PND. CPI-17, PKC-potentiated inhibitory protein of protein phosphatase-1; Mypt1, myosin phosphatase targeting subunit-1; MLCK, myosin light chain kinase; Adra1a, α1a-adrenergic receptor.