Fig. 1.
Seahorse experimental design. Determinations of the various parameters of oxygen consumption rate (OCR), expressed as pM·min−1·μg protein−1, are shown schematically. The Roman numerals show five different measurement cycles, and the vertical arrows demonstrate the injection of diazoxide/DMSO/l-NAME, oligomycin, FCCP, antimycin, and rotenone. The gray boxes represent the different parameters that were calculated to characterize mitochondrial bioenergetics in cerebral arteries: basal respiration, ATP production, proton leak, maximal respiration, nonmitochondrial respiration, and spare capacity.