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. 2015 Sep 14;309(9):H1528–H1542. doi: 10.1152/ajpheart.00070.2015

Fig. 10.

Fig. 10.

Knockdown of RACK1 in SHR CFs. A: Western blots for RACK1 (36 kDa) and α-tubulin (50 kDa; loading control) expression in SHR CFs subjected to either a nontargeting short hairpin (sh)RNA lentiviral vector or a RACK1 shRNA lentiviral vector. Some CFs were pretreated (Tx) with NPY1–36 (10 nmol/l) plus sitagliptin (1 μmol/l) for 3 days, whereas other CFs were not pretreated (No Tx). B: densitometry results from the blots shown in A. C: effects of NPY1–36 (10 nmol/l) plus sitagliptin (1 μmol/l) for 3 days on SHR CF number in cells pretreated with either nontargeting shRNA or RACK1 targeting shRNA. a,bSignificantly different (by Fisher's LSD test) from the corresponding nontargeting shRNA (a) or No Tx (b). Sample sizes (n individual experiments) are given shown.