Fig. 5.

A: dipeptidyl peptidase 4 (DPP4) protein expression in SHR and WKY CFs. Western blot analysis revealed the expression of DPP4 protein in SHR and WKY CFs, as evidenced by the detection of previously reported DPP4 bands (220 kDa, DPP4 dimer; 110 kDa, DPP4 monomer; 70 kDa, lower-molecular-weight DPP4 fragment). Expression of DPP4 protein was similar in WKY and SHR CFs, as quantified by densitometric analysis of Western blots (normalized to α-tubulin as a loading control). B: Y₁ receptor protein expression in SHR and WKY CFs. Western blot analysis revealed the expression of Y₁ receptor protein in WKY and SHR CFs, as evidenced by detection of previously reported Y₁ receptor band at 70 kDa. Expression of Y₁ receptor protein was similar in WKY and SHR CFs, as quantified by densitometric analysis of Western blots (normalized to α-tubulin as a loading control). Sample sizes (n individual samples) are shown.