Fig. 8.
Effects of NPY1–36 and PYY1–36 on the expression of collagen mRNA in WKY (A–C) and SHR (D–F) CFs. CFs were cultured to confluence in DMEM-F-12 containing 10% FBS under standard tissue culture conditions. Cells were rendered quiescent with DMEM containing 0.4% BSA for 48 h. Collagen synthesis was initiated by treating growth-arrested cells for 48 h with culture medium supplemented with PDGF (25 ng/ml) and containing either no treatments or 10 nmol/l of either NPY1–36 or PYY1–36. RNA was extracted, and real-time quantitative PCR was performed. Sample sizes (n individual samples) are shown. Ct, threshold cycle.