Fig. 5.

Effect of FSS exposure on PLGF levels in HCAEC monocultures. A: baseline (unstimulated) level of PLGF protein was significantly lower in medium of HCAEC monocultures than in medium of HCAEC-HCASMC cocultures (n = 5). *P < 0.001. B: HCAEC monocultures were exposed to static conditions, normal FSS, or high FSS for 2 h as described for cocultures. Culture medium was sampled before and after shear exposure. High FSS and normal FSS induced a significant increase in PLGF protein, but only at 8 h after exposure (n = 5). *P < 0.05. Thus the time course and intensity dependence of the effect were altered in the absence of HCASMC.