Fig. 1.

N-acetylcysteine (NAC) reduces ERK1/2 phosphorylation but not β-myosin heavy chain expression. A: tropomyosin (Tm)-E180G mice display increased ERK1/2 phosphorylation, which is reduced to nontransgenic (NTg) levels with NAC administration. B: representative Western blot images of phosphorylated (p)-ERK1/2 and total ERK1/2. C: Tm-E180G mice have increased β-myosin heavy chain expression, which is not reduced with NAC administration. D: representative 6% SDS-PAGE gel image showing β-myosin heavy chain expression. Data were analyzed by 2-way ANOVA, followed by Tukey's post hoc test for multiple comparisons and represented as means ± SE; n = 3–6 hearts/group. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001.