Figure 7. Altered cytosolic calcium transient in dysferlin‐deficient cardiomyocytes .

A, when stimulated with 3 or 10 nm isoproterenol, the level of resting cytosolic calcium in dysferlin knockout cardiomyocytes during paced contractions showed a trend of lower levels as compared with that in wild‐type cells, although statistical significance was not established. B–D, the peak cytosolic calcium (B), time to peak calcium (T_P) (C) and time to 25% calcium re‐sequestration (TR₂₅) (D) were not different between dysferlin knockout and wild‐type cardiomyocytes. E, the time to 75% calcium re‐sequestration (TR₇₅) was significantly longer in dysferlin knockout cardiomyocytes than that in wild‐type cells at baseline and under 3 or 10 nm isoproterenol treatment (*P<0.05 in Student's t test). Isoproterenol at 30 nm diminished the difference.