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. 2015 Dec 2;6:1063. doi: 10.3389/fpls.2015.01063

FIGURE 2.

FIGURE 2

Expression level of genes encoding SAMDC, ADC, SPDS and SPMS during SE in ‘Xinluzao 33’. The equation 2-ΔCt was applied to calculate the relative expression level using GhUBI as the reference gene. (A–I) Relative expression level of GhSAMDC1/2/3/4, GhADC1/2/3, GhSPDS and GhSPMS. (A) Relative expression level of GhADC1. (B) Relative expression level of GhADC2. (C) Relative expression level of GhADC3. (D) Relative expression level of GhSAMDC1. (E) Relative expression level of GhSAMDC2. (F) Relative expression level of GhSAMDC3. (G) Relative expression level of GhSAMDC4. (H) Relative expression level of GhSPDS. (I) Relative expression level of GhSPMS. Abbreviations on the x-axis: H, Hypocotyl; NEC, Non-embryogenic callus; EC, Embryogenic callus; EPED, Early phase of embryo differentiation; E, Embryo; RP, Regenerated plantlets. Values are the mean + standard error (n = 3).