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Immunofluorescence staining of LGR5 in neuroblastoma cell lines. (A) Immunofluorescence staining of Lgr5 in BE2c cells (B) Overlay shows that it is primarily located in the cytoplasm of BE2c neuroblastoma cells (C) NB69 cells show an absence of Lgr5 staining when stained under the same conditions as the BE2c cell line, indicating low expression (D) Overlay top right shows the morphology of the cell, with the absence of stain by phase contrast microscopy.
