Figure 9.
Survival of MTB decreases when HDAC1 is knocked down in macrophages. THP-1 monocytes (1 × 106 cells) seeded in a 6-well plate were treated with scrambled siRNA and siHDAC1, and infected with MTB. Macrophages not treated with siRNA but infected with MTB were also kept as control. The number of bacteria recovered from the macrophages 4 h after infection was considered as the number of bacteria that gained entry into the macrophages (and the number at 0 h for studying the viability inside macrophages); and subsequent isolation of bacilli from the macrophages was carried out at 24, 48, and 72 h. The number of viable bacilli in each of the wells was assayed by plating on a 7H10 agar plates and incubating the plates at 37°C, and counting the colony forming unit (cfu). Data shown here are the mean ± SD of three independent experiments.