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Amplicon melting analysis of ampilcon EST-66. A total of 96 peanut varieties were analyzed by HRM in ampilcon EST-66. Of the 96 varieties, 28 samples showed red line, and remaining 68 samples showed in gray lines. Representative sequence traces were shown on the right; homozygote at the top and heterozygote at the bottom. Vertical arrows showed the SNP position.
