Table 1.
Marker | Primer sequence | Variant classes | Allele frequency | Polymorphic information content (PIC) | ||
---|---|---|---|---|---|---|
Forward | Reverse | Allele 1 (%) | Allele 2 (%) | |||
EST-11SNP | TTCACGCCAGATGAGGAT | CAGGAGTCGGGCTATTGT | C/G | 4 | 92 | 0.080 |
EST-12SNP | CATCTCAGCACTCAACTT | GAGTAAGGGTTTGAGGAA | C/T | 1 | 95 | 0.021 |
EST-21SNP | TTGAGTGAACCAGCTTGAGG | GCCGACGAAGAAGAGGAATA | G/T | 15 | 81 | 0.264 |
EST-26SNP | TACCCTTGACTTGGTTTATGGC | GCTCTTCAGCGAACTGTCCC | A/C | 8 | 88 | 0.153 |
EST-33SNP | CAAACAAAGTCATCGCAGTC | CTCTTCTATCCCATCTCACAG | G/T | 92 | 4 | 0.080 |
EST-45SNP | AACTGGCGTCTTCTTCACCG | GTCCCAAGCCTGCATCCAAA | G/T | 88 | 8 | 0.153 |
EST-48SNP | ACCGCTTACTATCACTACCA | TCTAATATGACTGTTCCCAGA | G/T | 83 | 13 | 0.234 |
EST-66SNP | CATCGTCATCCGTGAGGTGT | TTGGACTTGAGGAGGAGGTAG | A/G | 28 | 68 | 0.413 |
EST-70SNP | AGGCGTTGAAATGCAGGTCC | GGCATCCCAGTTCCAGAAGG | C/T | 79 | 17 | 0.291 |
EST-80SNP | ACAGAGTCATTGGTGATGGGAGTT | CACTTGCAGGTGCATGGGTT | A/G | 5 | 91 | 0.099 |
EST-87SNP | ATGAGCTGGACCAGATGGAG | CCTGAAGTTACACTGTTGAGGC | A/G | 13 | 83 | 0.234 |
EST-92SNP | GAGTAATCGTTGAGAAACTCGC | TCAGAAGACTTCGCCACCTT | A/T | 2 | 94 | 0.041 |
GSS-2SNP | ACTTAGCATAAGAGGGTATTTG | AGCGGAACAGGATAAGCAAT | A/G | 14 | 82 | 0.249 |
GSS-10SNP | GCCAGAACTATGCTTCCCTT | AAATCCCTCTGAGGACAATC | A/C | 21 | 75 | 0.234 |
GSS-26SNP | GACTCAGCGCAGTGCCAACT | GTGCCATGCTTACTCAACAAAA | C/A | 85 | 11 | 0.203 |
GSS-35SNP | ATTGAACAAAGGATTGAGAT | CAAATGAAGGTGTAGATGGC | A/T | 24 | 72 | 0.375 |
GSS-44SNP | CAACATCCAGACACCCCAAAT | CTCCAGGTCAGGCTGAACAT | A/C | 16 | 80 | 0.278 |
GSS-45SNP | TCTAGCCAGATCGGCCATAC | GAATACGTGACTGACCCAAG | A/G | 87 | 9 | 0.170 |
GSS-46SNP | TCTACCTTTGCCTTATCCAC | ACTTGTTCTGAAAAGATGCC | C/T | 4 | 92 | 0.080 |
GSS-49SNP | TTCAATGCTCAATCATTCCCACTA | GAGGACTTGTCCGCCACCTT | A/C | 18 | 78 | 0.305 |
GSS-52SNP | AAGCCTTAAACAGGGGTAGC | TTGGTAGGAGGTGAGCGATG | C/T | 89 | 7 | 0.135 |
GSS-54SNP | AGTGCAAAGGTTGCGTCTTG | TTGCTCCATTTTCATTAGGTTTT | C/T | 82 | 14 | 0.249 |
GSS-56-1SNP | ACATTGGCAGGTGGTGGTAA | ATGGGAGAAATGTCACTTTATGGT | A/G | 6 | 90 | 0.117 |
GSS-56-2SNP | TTCTGTATGCAAATACTCCG | ATGAATACTACTGCTTCCTG | C/T | 15 | 81 | 0.264 |
GSS-67-1SNP | ATAATCCACTGCCACCAGAA | TATGCTATGCCTCAACAAGG | C/T | 87 | 9 | 0.170 |
GSS-67-2SNP | ATGGTTCATCCCAAAGATAG | TGAGATTCATACCCAAAGAG | C/T | 83 | 13 | 0.234 |
GSS-69SNP | TGGCTAGAGGATGGTTGGAG | TGATAGCCCGGTCTATGGTA | A/C | 26 | 70 | 0.395 |
GSS-72SNP | CATTCTACTGGTGGGTCTGT | CATGAACTGGATTATTTGCA | A/T | 71 | 15 | 0.429 |
GSS-74SNP | ATTTGCCACTAACTCCCTTC | TTAGCATACAAGATAGTTGAAAGT | C/T | 89 | 7 | 0.135 |
GSS-76SNP | ACAAGCTATCCCAACTCCAC | TAAGCAGTCCACCAATCAAA | A/G | 33 | 61 | 0.478 |
GSS-80SNP | CCTAGAATTATTCATAACCTCCAT | GCTTACACGTCACATGCTTT | A/G | 17 | 79 | 0.291 |
GSS-81SNP | CCATTACTTGTTTTGCTCAC | TGTGGTTATAGAACAGAGGG | C/T | 83 | 13 | 0.234 |
GSS-99SNP | TAGAAAGCCCTGGATGTTAG | AAAGAGTGCAGATGCTGTCA | C/T | 90 | 6 | 0.117 |