Table 1.
Bacterial strains and plasmids used in this work.
| Strain or construct | Description | Reference |
|---|---|---|
| Escherichia coli strains | ||
| HB101 | F-, hsdS20(rB-mB-), recA13, ara14, proA2, lacY1, galK2,rpsL20 (SmR) xyl-5, mtl-1, supE44, λ-, | Figurski and Helinski, 1979 |
| SY327 | Δ(lac pro) argE(Am) recA56 rif R nalAλ pir | Miller and Mekalanos, 1988 |
| DH5α | F- Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17(rK- mK+) phoA supE44 thi-1 gyrA96 relA1λ- | Hanahan, 1983 |
| Stenotrophomonas maltophilia strains | ||
| SMK279a | Clinical isolate from the blood of a cancer patient | Avison et al., 2000 |
| SMK279aΔsmlt1134 | SMK279a lacking the smlt1134 gene coding for a putative DNA transport competence protein | This study |
| SMK279aΔsmlt2851Δ smlt2852 | SMK279a lacking the smlt2851 and smlt2852 genes coding for putative transmembrane efflux proteins | This study |
| SMK279aΔsmlt3723 | SMK279a lacking the ampR (smlt3723) gene; a putative HTH and LysR family transcriptional regulator | This study |
| SMK279aΔsmlt0387 | SMK279a carrying a deletion in smlt0387 | This study |
| SMK279a EM2 | SMK279a carrying pBBR1MCS-5::PblaL2::rfp | This study |
| SMK279a EM3 | SMK279a carrying pBBR1MCS-5::PblaL1::rfp | This study |
| SMK279a EM4 | SMK279a carrying pBBR1MCS-5::Pless::rfp | This study |
| SMK279a EM5 | SMK279a carrying pBBR1MCS-5::PblaL2::cfp | This study |
| SMK279a EM6 | SMK279a carrying pBBR1MCS-5::PblaL2::yfp | This study |
| SMK279a EM7 | SMK279a carrying pBBR1MCS-5::PblaL2s::rfp | This study |
| SMK279a EM8 | SMK279a carrying pBBR1MCS-5::PblaL2::rfp::smlt1134 | This study |
| SMK279a EM9 | SMK279a carrying pBBR1MCS-5::PblaL2::rfp:: smlt2851::smlt2852 | This study |
| CF148 | Clinical isolate from the respiratory tract of a cystic fibrosis patient | Jörg Steinmann (Universitätsklinikum Essen) |
| DSM-50170 | Reference strain from the oropharyngeal region of a patient with cancer | Leibniz institute DSMZ |
| Plasmids | ||
| pRK2013 | KanR; RK2-derived helper plasmid carrying the tra and mob genes for mobilization of plasmids containing oriT | Figurski and Helinski, 1979 |
| pBBR1MCS-5 | Broad host range vector, low copy, Gmr | Kovach et al., 1995 |
| pBBR1MCS-5::rfp | pBBR1MCS-5 carrying the rfp gene in the MCS | This study |
| pBBR1MCS-5::PblaL1::rfp | PblaL2::rfp reporter fusion in pBBR1MCS-5 | This study |
| pBBR1MCS-5::PblaL2::rfp | PblaL1::rfp reporter fusion in pBBR1MCS-5 | This study |
| pBBR1MCS-5::PblaL2::yfp | PblaL2::yfp reporter fusion in pBBR1MCS-5 | This study |
| pBBR1MCS-5::PblaL2::cfp | PblaL2::cfp reporter fusion in pBBR1MCS-5 | This study |
| pBBR1MCS-5::Pless::rfp | Promoterless rfp reporter fusion in pBBR1MCS-5 | This study |
| pBBR1MCS-5::PblaL2s::rfp | PblaL2s::rfp reporter fusion constructed with the ampR-blaL2 intergenic region in pBBR1MCS-5 | This study |
| pBBR1MCS-5:: PblaL2::rfp::smlt1134 | smlt1134 gene under its native promoter cloned into the pBBR1MCS-5::PblaL2::rfp reporter fusion | This study |
| pBBR1MCS-5::PblaL2::rfp:: smlt2851::smlt2852 | Putative operon of smlt2851 and smlt2852 under its native promoter cloned into the pBBR1MCS-5::PblaL2::rfp reporter fusion | This study |
| pGPI-SceI-XCm | CmR, TpR; mobilizable suicide vector; carries the R6Kγ origin of replication, the I-SceI recognition site and a xylE reporter gene | Hamad et al., 2010 |
| pDAI-SceI-SacB | TetR; mobilizable broad host range plasmid; carries the gene for the I-SceI homing endonuclease and the sacB gene | Flannagan et al., 2008; Hamad et al., 2010 |
| pUDK011 | pGPI-SceI-XCm with a 705-bp SphI/KpnI insert of SMK279a containing the flanking region upstream of smlt1134 | This study |
| pUDK012 | pUDK011with a 741-bp KpnI/XbaI insert of SMK279a containing the flanking region downstream of smlt1134 | This study |
| pUDK014 | pGPI-SceI-XCm with a 779-bp NotI/KpnI insert of SMK279a containing the flanking region upstream of smlt2852 | This study |
| pUDK015 | pUDK014 with a 776-bp KpnI/XbaI insert of SMK279a containing the flanking region downstream of smlt2851 | This study |
| pUDK017 | pGPI-SceI-XCm with a 761-bp NotI/KpnI insert of SMK279a containing the flanking region upstream of smlt3723 | This study |
| pUDK018 | pUDK017 with a 660-bp KpnI/XbaI insert of SMK279a containing the flanking region downstream of smlt3723 | This study |