Figure 3.

Expression of Kv7.2 in neuromatose fiber endings 15 day post-axotomy. (A) Sciatic nerve branches from SNI mice were sectioned and stained with Dapi. Injured fibers from common peroneal and tibial nerves (forming the neuroma) and un-injured fibers from sural nerve (delineated by dashed lines) are anatomically separated and can be readily identified. (B–E) images correspond to a magnification of the un-injured sural nerve. Sciatic nerves from SNI mice were sectioned, immunostained with Kv7.2 antibody and counterstained with Dapi to label cell nuclei. In sural intact branches labeling was found in normal nodes, indicated by white arrows in (B–D), and was not associated with cell nuclei (C–E). (F–M) images correspond to a magnification of injured common peroneal and tibial nerves. (F–I) Injured fibers show Kv7.2 in large structures which were surrounded by nuclei. (J–M) Show sciatic nerve sections from SNI mice immunostained with Kv7.2 and counterstained with NeuroTrace showing that Kv7.2(+) accumulations were localized in terminal axoplasm. Scale bar in (M) applies to (E,I) images. Scale bar in (L) applies to the rest of images.