Skip to main content
. 2015 Dec 2;5:17397. doi: 10.1038/srep17397

Figure 1. OTC-∆ is rapidly degraded in mitochondria in an ATP-dependent manner.

Figure 1

(A) Import of [35S]OTC and [35S]OTC-∆ preproteins into isolated mitochondria (7.5 min) with and without membrane potential and trypsin treatment as indicated. Import was analysed by digital autoradiography following separation by SDS-PAGE. (B) Turnover of [35S]OTC-∆ (lanes 7–12) relative to [35S]OTC (lanes 1–6) in mitochondria analysed by import-chase assay (top panel). The bottom left panel shows a representative digitial autoradiogram of the assay following separation by SDS-PAGE while the right panel is a quantification of four independent experiments. (C) Turnover of [35S]OTC-∆ in mitochondria analysed by import-chase assay with high (↑) or low (↓) ATP levels. Samples were analysed by digital autoradiography following separation by SDS-PAGE (representative strip is shown in bottom left panel) and quantitation of three independent experiments (right panel). Error bars shown in Fig. 1 represent standard error of the mean (SEM). p, preprotein; i, processing intermediate; m, mature protein.