Table 1.

2D Gel	Protein Identification	Exaiptasia	Protein	Protein	Fold	P
Spot		Genome	MW			value
Number		Gene ID	(Da)	PI	Change	T test
2	Heat Shock Protein (70 KDa)	AIPGENE12496	74,685	5.6	2.02	0.091
6	Signal Recognition Particle	AIPGENE24594	72,108	6.4	1.88	0.12
84	MRP Protein	AIPGENE1468	95,674	9.3	1.72	0.029
32	Calumenin-A	AIPGENE25880	35,247	4.5	1.69	0.015
34	Glutamate Receptor	AIPGENE24462	101,286	9.2	1.63	0.0081
50	Myosin Heavy Chain	AIPGENE8264	220,878	5.4	1.63	0.022
27	Aminopeptidase	AIPGENE26826	53,551	6.4	1.59	0.0046
3	Zona Pellucida	AIPGENE843	47,035	5.0	1.57	0.044
11	Heat Shock Protein (60 KDa)	AIPGENE15267	62,708	5.3	1.57	0.051
13	Moesin/ezrin/radixin	AIPGENE9804	66,884	5.8	1.56	0.064
43	Fructose-Bisphosphate Aldolase	AIPGENE2871	38,732	7.6	1.50	0.055
61	Heat Shock Protein (70 KDa)	AIPGENE12775	41,848	5.4	1.47	0.067
57	Voltage Dependent Anion Selective Channel	AIPGENE3808	35,188	9.1	1.45	0.027
82	Rho GDP-Dissociation Inhibitor	AIPGENE26434	22,251	4.8	1.32	0.12
65	Cathepsin	AIPGENE26157	36,124	6.7	1.25	0.041
42	Aspartate Aminotransferase	AIPGENE19338	45,969	6.8	−1.47	0.0043
87	Zinc Finger Protein	AIPGENE28354	68,856	8.8	−1.48	0.015
35	Cysteine Desulfurase	AIPGENE22361	54,104	6.2	−1.48	0.091
39	Fumarylacetoacetase	AIPGENE8362	46,370	6.2	−1.49	0.013
15	Selenium Binding Protein	AIPGENE13749	53,877	5.9	−1.50	0.0025
28	Cysteine Desulfurase	AIPGENE22361	54,104	6.2	−1.50	0.0046
14	Bleomycin Hydrolase	AIPGENE9335	55,335	5.8	−1.53	0.015
41	Pancreatic Triacylglycerol Lipase	AIPGENE19570	38,517	8.7	−1.55	0.025
71	Heat Shock Protein (70 KDa)	AIPGENE8252	41,986	5.3	−1.58	0.055
46	Calumenin B	AIPGENE9938	38,837	5.5	−1.59	0.0044
38	Cysteine Desulfurase	AIPGENE22361	54,104	6.2	−1.69	0.0021
56	Hemicentin	AIPGENE28714	49,371	6.6	−1.70	0.011
55	Thyroglobulin	AIPGENE20635	314,272	8.6	−1.85	0.0039
74	Nuclear Receptor	AIPGENE629	49,435	5.4	−1.91	0.046
70	Heat Shock Protein (70 KDa)	AIPGENE8252	41,986	5.3	−2.67	0.0018
68	Cyclic AMP and cGMP Phosphodiesterase	AIPGENE4644	51,903	5.8	−4.05	0.017
69	Heat Shock Protein (70 KDa)	AIPGENE8252	41,986	5.3	−9.73	0.0037

Identification of the 32 proteins subjected to Mass Spectrometry analysis. 2D Gel Spot Number: Number as indicated on the 2D-DIGE gel on supplemental Fig. 4; Protein Identification: Identity assigned by BLAST data base searches; Exaiptasia Genome Gene ID: Gene ID is based on the gene mapping identification for the Exaiptasia genome (aiptasia.reefgenomics.org⁷³); Protein MW: Molecular weight of the protein; Protein PI: Isoelectric point of the protein; Fold Change: The fold change is based on protein expression of primed anemones versus non-primed (naïve) anemones; P Value T test: The significance probability value based on statistical T test.