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. 2015 Sep 25;9(6):606–612. doi: 10.4162/nrp.2015.9.6.606

Fig. 2. A water-soluble fraction of Smilax china L. leaf ethanol extract (wsSCLE) treatment inhibits lipid accumulation in 3T3-L1 adipocytes.

Fig. 2

Differentiation was induced in 3T3-L1 cells 2 days after they attained confluence. Cells were then treated with wsSCLE (0.1 and 0.25 mg/mL) for 9 days. At the end of the treatment period, lipid content was analyzed using Oil Red O staining. (A) Representative images of Oil Red O staining. (B) Quantification of lipid accumulation based on the optical density values at 520 nm of destained Oil Red O extracted from the 3T3-L1 cells. Data are expressed as mean ± SD of triplicate experiments. # P < 0.05 compared to control cells (white bar), * P < 0.05 compared to adipocytes.