Figure 1. miRNA microarry analysis and qRT-PCR validation of microarry results.
(a) Heat map representation of the miRNA microarray analysis in normal fertility controls and severe asthenozoospermia using Applied Biosystems Chip. (b) qRT-PCR validation of TLDA screening results. (c) qRT-PCR validation of stage 1 for microarry results. (d) qRT-PCR validation of stage 2 for microarry results. The seminal plasma expression levels of miR-151a-5p,miR-101-3p and let-7b-5p were significantly different between NF and SA (P < 0.05). NF, Normal Fertility; SA, Severe Aasthenozoospermia. Asterisks denote significant differences from controls (P < 0.05).