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. 2015 Dec 2;5:17463. doi: 10.1038/srep17463

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Copyright © 2015, Macmillan Publishers Limited

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Testes from male flies expressing a Su(var)3-9 RNAi transgene with the germline specific nanos-Gal4 were immunostained for Vasa (magenta cytoplasmic staining) and for fusomes and hub cells (both red). Asterisk marks the position of the hub. Scale bar = 20 μm. In control testes (which did not express Su(var)3-9 RNAi) 5 days after clone induction, clonal GFP+ cells were found as both GSCs (next to the hub) and spermatogonia. Five days after Su(var)3-9 RNAi was expressed, no GFP+ GSCs were observed next to the hub, although GFP+ spermatogonia were still observed.