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. 2015 Dec 2;5:17463. doi: 10.1038/srep17463

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Copyright © 2015, Macmillan Publishers Limited

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Testes from 3-day old hop²⁵/Y male survivors of indicated genetic backgrounds were stained with DAPI, anti-Vasa, mAb3A9 and anti-FasIII, as indicated. (A) A hop²⁵/Y; nos-Gal4 testis showing no sperm bundles, with all Vasa-positive germ cells exhibiting large nuclei (arrow) and dotted fusome but lacking intense DAPI signals. (B) A partially rescued hop²⁵/Y; nos-Gal4/UAS-HP1 testis. Thick sperm bundles were seen (left). A fraction of Vasa-positive germ cells contain small DAPI-dense nuclei, dotted fusome, resembling GSCs (arrow), were observed. (C) A completely rescued testis from a hop²⁵/Y; nos-Gal4/UAS-HP1 male survivor that had become fertile. The testis resembles that of wild-type flies, with mature long sperm bundles.