Table 1. Su(var)3-9 is essential for GSC self-renewal.
| Su(var)3-92 mutant clones (GFP–) | |||
|---|---|---|---|
| Days after Induction | Clone Genotype | Testes with GSCclones/testes scored | testes scored |
| 2 | Wild type | 24/42 (57%) | 8/42 (19%) |
| Su(var)3-92 | 15/48 (31%) | 19/48 (40%) | |
| 5 | Wild type | 24/44 (55%) | 9/44 (20%) |
| Su(var)3-92 | 0/42 (0%) | 13/42 (31%) | |
| 7 | Wild type | 31/53 (58%) | 4/53 (7.5%) |
| Su(var)3-92 | 0/30 (0%) | 6/30 (20%) | |
| Su(var)3-9 RNAi clones (GFP+) | |||
| Days after Induction | Clone Genotype | Testes with GSCclones/testes scored | Testes with non-GSC clones/testes scored |
| 2 | Wild type | 62/67 (92%) | 5/67 (7.5%) |
| Su(var)3-9 RNAi | 4/74 (5.4%) | 44/74 (59%) | |
| 5 | Wild type | 44/51 (81%) | 6/51 (12%) |
| Su(var)3-9 RNAi | 3/65 (4.6%) | 18/65 (28%) | |
| 7 | Wild type | 35/46 (76%) | 10/46 (22%) |
| Su(var)3-9 RNAi | 1/27 (3.7%) | 5/27 (19%) | |
Mutant clones homozygous for Su(var)3-92 (loss-of-function allele) or wild-type (control) were identified as GFP– cells. Knock-down clones expressing Su(var)3-9 RNAi or wild-type control were identified as GFP+ cells. Testies with one or more mutant clones at the GSC position were counted as “testes with GCS clones”.