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. 2015 Dec 2;5:17587. doi: 10.1038/srep17587

Figure 1. Expression of bHLH129 is negatively regulated by ABA.

Figure 1

(A) Expression of bHLH129 in Arabidopsis seedlings in response to ABA treatment. RNA was isolated from ABA treated Arabidopsis seedlings and RT-PCR was used to examine the expression of bHLH129. Expression of ACT2 was used as a control. (B) Quantitative RT-PCR (qRT-PCR) analysis of bHLH129 expression in response to ABA, methyl jasmonate (MeJA), and 2,4-epibrassinolide (EBL) treatments. RNA was isolated from ABA, methyl jasmonate, or 2,4-epibrassinolide treated Arabidopsis seedlings, and qRT-PCR was used to examine the expression of bHLH129. Expression of ACTIN2 was used as a reference gene, and expression of bHLH129 in the absence of ABA was set as 1. Data represent the mean ± standard deviation (SD) of three replicates. (C) Expression of bHLH129 in aba1-5 mutants. RNA was isolated from 12-day-old Col and aba1-5 mutant seedlings and qRT-PCR was used to examine the expression of bHLH129. Expression of ACT2 was used as a reference gene, and expression of bHLH129 in Col wild type was set as 1. Data represent the mean ± standard deviation (SD) of three replicates.