Table 2. Survival rate and tissue distribution of novel H9N2 and H5 avian influenza viruses in mice.
| Virus | Survival rate of mice | Virus replication in experimentally infected mice Virus titers in organs of mice (log10 EID50/ml) |
|||
|---|---|---|---|---|---|
| Tissue | 3 day | 6 day | 9 day | ||
| A/chicken/Zhejiang/C1/2013(H9N2) | 100% (6/6) | Lung | 2.5 ± 0.71 | 3.5 ± 0.71 | 2.0 ± 0 |
| Brain | – | ||||
| Heart | – | – | – | ||
| Liver | – | – | – | ||
| A/quail/Zhejiang/2A2/2013(H9N2) | 100% (6/6) | Lung | 2.0 ± 0.0 | 3.5 ± 0.71 | 2.5 ± 0.71 |
| Brain | – | – | |||
| Heart | – | – | – | ||
| Liver | – | – | – | ||
| A/goose/Zhejiang/112071/2014(H5N1) | 83.33% (5/6) | Lung | 3.5 ± 0.71 | 4.5 ± 0.71 | 4.0 ± 0 |
| Brain | – | ||||
| Heart | – | – | – | ||
| Liver | – | – | – | ||
| A/goose/Zhejiang/77166/2014(H5N2) | 66.67% (4/6) | Lung | 4.0 ± 0 | 5.0 ± 0 | 4.5 ± 0.71 |
| Brain | – | – | – | ||
| Heart | – | – | – | ||
| Liver | – | 3.5 ± 0.71 | – | ||
| A/goose/Zhejiang/925105/2014(H5N6) | 100% (6/6) | Lung | 3.5 ± 0.71 | 4.5 ± 0.71 | 3.0 ± 0 |
| Brain | – | ||||
| Heart | – | – | – | ||
| Liver | – | – | – | ||
| A/goose/Zhejiang/925037/2014(H5N8) | 83.33% (5/6) | Lung | 3.0 ± 0 | 5.0 ± 0 | 3.5 ± 0.71 |
| Brain | – | – | – | ||
| Heart | – | – | – | ||
| Liver | – | – | – | ||
Notes: Fifteen (15) 6-week-old female BALB/c mice were intranasally inoculated with 106.0 EID50 of virus. Three (3) mice each were sacrificed on days 3, 6, and 9 post-inoculation. The lungs, brain, heart, and liver were collected for virus titration in embryonated chicken eggs. The survival rate was determined in the remaining six mice 14 days following inoculation. The EID50 was determined in embryonated chicken eggs using the method published by Reed and Muench. Values represent mean ± SD.