Figure 8. Expression profiles of the six stress–responsive genes in wild type (WT), transgenic lines (TG8 and TG10) before and after cold treatment.

RNA was extracted from leaves sampled at the onset and before after 3 d of cold stress, and reverse transcribed to synthesize cDNA, which was used for RT–PCR analysiswith primers specific for these genes. mRNA levels of these geneswere normalized to the transcripts of Actin in the same samples.