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. 2015 Dec 2;5:17626. doi: 10.1038/srep17626

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Copyright © 2015, Macmillan Publishers Limited

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HEK293T cells were serum-starved for 18 h, preincubated with or without Fumos (20 μM 24 h), and then treated with EGF (20 ng/ml, 5 min). (a) Shp2 was immunoprecipitated from cell lysate supernatants. (b) Gab1 was immunoprecipitated from cell lysate supernatants. Immunoprecipitates were analyzed by immunoblotting with antibodies to Gab1, RasGAP, pY and Shp2. (c) Densitometric analysis of p-Gab1/Shp2 ratio in the Western blots. **p = 0.002 < 0.01/F = 37 vs EGF. (d) Densitometric analysis of RasGAP/Gab1 ratio in the Western blots. **p < 0.01/F = 30 vs EGF, one-way ANOVA followed by Tukey’s test. Bars represent mean ± S.E.M. values from three experiments.