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. Author manuscript; available in PMC: 2016 Nov 25.
Published in final edited form as: Adv Funct Mater. 2015 Nov 25;25(44):6939–6850. doi: 10.1002/adfm.201503047

Figure 3.

Figure 3

Low-CPA vitrification of stem cells using quartz microcapillary (QMC). (A) A typical picture showing vitrification (clear appearance or visible ice formation) of VSQ but not medium in QMC by plunging the QMC into liquid nitrogen (LN2) for cooling. VSQ: Vitrification solution for QMC which is mESC or hADSC medium with 1.5 M 1,2-propanediol (PROH, penetrating) and 0.5 M trehalose (non-penetrating). The QMC with an opaque appearance on the left is filled with cell culture medium without any CPA. (B) Viability of mESCs and hADSCs either with (W/) or without (W/O) alginate hydrogel encapsulation (Encap) before and after vitrification in VSQ. **: p < 0.01. (C) Typical phase and fluorescence images showing the viability of mESCs before and after vitrification in VSQ. Scale bar: 200 μm.