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. Author manuscript; available in PMC: 2016 Nov 25.
Published in final edited form as: Adv Funct Mater. 2015 Nov 25;25(44):6939–6850. doi: 10.1002/adfm.201503047

Figure 7.

Figure 7

Alginate hydrogel microencapsulation inhibits devitrification during warming. Polarized (color) and phase contrast (grayscale) images at −60 °C at the end of cooling and at −7 and −15 °C during warming with annealing for 20 min for an alginate hydrogel microcapsule in mESC medium without (A) and with (B) CPA (1.5 M PROH and 0.5 M trehalose), respectively. No visible ice formation inside the microcapsule is observable during both cooling and warming while the fine ice crystals (FIC, *) outside the microcapsules in the bulk solution merge into large ice crystals (LIC, **) during warming. Scale bar: 100 μm.