Figure 1.

Five consecutive daily treatments with parachlorophenylalanine (PCPA) maximally decreased blood and brain 5-hydroxytryptamine (5-HT). (A) 5-HT levels in forebrain and medulla of PCPA-treated mice (800 mg/kg for 5 days) and Lmx1b^f/f/p mice as a percentage of saline treated control mice (n = 7 all groups). *P < 0.001. (B) Blood and forebrain 5-HT reached a minimum following the fifth day of PCPA treatment (blood, n = 5 for each day; cortex day 0, n = 10; day 3, n = 5; day 5, n = 11; day 7, n = 5). For all points compared to day 0: P < 0.001; for blood and forebrain on day 3 compared to day 5: P < 0.05. Data are shown as mean ± standard error of the mean. Unpaired t-test with Welch correction.