Fig. 3.

Schematic overview of the GigaTON pipeline search, browse and retrieve tool. The database can be used to investigate the transcriptome assembly by searching contigs (orange) or polymorphism variants (blue), or to download featured data sets and library BAM files (black). The search for a specific contig (or a contig subset) can be achieved either by differential analysis of library pools (Venn diagrams and Digital Differential Display (DDD)), either by BLAST or BioMart search. The BioMart portal (grey) enables to search a dataset using a combination of several filter criteria (shown in white), and to retrieve the results featuring a wide range of information characteristics and parameters, i.e. ‘attributes’ (shown in yellow). The resulting dataset (Selected contigs, red; Selected variants, shonw in turquoise) and associated attributes can be downloaded and/or browsed within the GigaTON pipeline for additional information. The latter include Sequence info (translation frames, ORF length…) or Depth (detailed view of expression level and assembly variants between libraries) for contigs (brown), or allele and feature views for variants (shown in purple). The number of user-exploitable fields/buttons is indicated in brackets under each corresponding criteria