Fig. 3.

MiR-152 regulated HLA-G expressions in BGC823 and SGC7901 cells. a. The binding sites of miR-152 on the wild-type or mutated 3’UTR sequences of HLA-G, wherein “CTG” and “ACT” were mutated to “AAA” and “GGA” respectively in Mut-HLA-G. b. The expression change of miR-152 in BGC823 and SGC7901 cells after transfected with miR-152 mimic or inhibitor for 12 h. c. HLA-G mRNA expression in miR-152 overexpressing or inhibiting cells after transfection for 24 h. d. Primers used for the construction of HLA-G luciferase reporter (PGL3-HLA-G) within HLA-G 3'-UTR sequences. Mutation sites within Mut-HLA-G sequences were indicated with rectangle boxes. e. HLA-G 3’-UTR activity change resulted from miR-152 dysregulation through luciferase assay. The results were normalized to those of Renilla luciferase activities, and statistically analyzed as compared to that from the cotransfection with negative miRNA. f. Detection for Mut-HLA-G 3’-UTR activity change. g. HLA-G level detection after miR-152 mimics or inhibitor transfection for 48 h. *P < 0.05; **P < 0.01