Fig. 2. : THP-1 derived macrophages treated with live or heat-killed (HK)Mycobacterium bovis bacillus Calmette-Guérin (BCG) produce chemokines. THP-1 derived macrophages were treated with intradermic or oral BCG Moreau in multiplicity of infection of 1/1 and 5/1 live or HK mycobacteria. Supernatants from 24 h and 72 h after treatment were removed and the detection of chemokines macrophage inflammatory protein (MIP)-1α and MIP-1β were analysed by enzyme-linked immunosorbent assay. Data are expressed as the mean and standard deviation (±) of three independent experiments. Asterisks mean statistical significance (*: p < 0.05; **: p < 0.008, Mann-Whitney U test).
