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. 2015 Nov 4;6:8820. doi: 10.1038/ncomms9820

Figure 6. The 3′ splice site sequence is critical for intron 2 retention of Aire gene.

Figure 6

(a) Comparison of 3′ splice site sequence of the Aire intron 2 with the consensus sequence. /, splice site. (b) Association between intron retention and 3′ splice site score revealed by comparing 57 retained introns with 188,151 unretained introns. (c) Effect of TTT mutation on intron 2 retention of minigene. Twenty-four hours after the transfection of WT and Jmjd6−/− MEFs with GAG- or TTT-type minigene, the ratio of mature to immature transcript was analysed by RT-PCR (n=3). Data are collected from three separate experiments and are expressed as mean±s.d. **P<0.01 (two-tailed Student's t-test). (d) Comparison of the 3′ splice site sequence among different species. Non-canonical sequences at PPT site are indicated in red.