Figure 3. CD39⁺CD8⁺ T cells exhibit Tc1 responsiveness.

(a) Healthy blood CD8⁺ T cells were stimulated with anti-CD3 (10 μg ml⁻¹) or/and CD28 (5 μg ml⁻¹) antibodies in the presence of vehicle or DPI (10 μM), and ROS induction was determined at 30 min. (b) Flow cytometric analyses of CD28 expression was done as based on expression of CD39 on CD8⁺ T cells (n=18); statistical analysis of percentages of two CD8⁺ T-cell subsets is shown in lower panel. (c) Flow cytometry of ROS induction, phospho-JNK and phospho-NFκB p65 in CD8⁺ T cells, stimulated with anti-CD3/CD28 antibodies for 30 min. Cells were pretreated with 4 μM of H₂DCFDA for ROS determination, as before. (d) Representative flow cytometric analyses of CD8⁺ T cells based on expression of CD39. CD8⁺ T cells were stimulated with anti-CD3/CD28 antibodies for 24 h. Statistical comparative analysis indicating different percentages of CD8⁺ T cells expressing IFNγ is shown in the right panel. (e) Flow analysis of CD226 (n=11) and CXCR3 (n=12) expression on CD39⁺CD8⁺ and CD39⁻CD8⁺ T cells. Data are presented as means±s.e.m., **P<0.01, ***P<0.001 (Student's t-test).