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. 2015 Jun 8;77(11):1443–1449. doi: 10.1292/jvms.15-0198

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©2015 The Japanese Society of Veterinary Science

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.

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Fig. 1. — Effects of LPS on CCh-induced contraction and time-dependent changes in COX-2 and iNOS gene expression by LPS. A: Inhibitory action of LPS on CCh-induced contraction. The sustained contraction 10 min after application of CCh (1 µM) was quantified. LPS (100 µg/ml) was pre-treated 0 to 4 hr before application of CCh. B-D: Effects of LPS on mRNA expression of COX-2 and iNOS. Typical results were shown in B from 4-8 experiments. Quantified results for COX-2 (C) and iNOS (D) were shown. Each symbol showed mean ± SEM. * or **P<0.05 or P<0.01, significantly different from control, respectively (n=5–9). Control means non-treated ileal tissue. The ileal tissue was incubated in the modified physiological salt solution (PSS) for 1–4 hr.