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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Tuberculosis (Edinb). 2015 Oct 19;95(6):689–694. doi: 10.1016/j.tube.2015.08.009

Figure 1. Characterization in vitro of Mtb strains with hoas deleted or disrupted by transposon insertion.

Figure 1

Figure 1

Figure 1

(A) Immunoblot of HOAS in lysates of WTCDC1551, hoas::tnCDC1551, Δ hoasCDC1551, hoas::tnCDC1551::hoas, and Δ hoasCDC1551::hoas using antiserum against HOAS. Antiserum against DlaT was used as a loading control. (B–E) Growth curves of WTH37Rv, ΔhoasH37Rv, and ΔhoasH37Rv::hoas in modified Sauton’s minimal medium supplemented with either (B) glycerol, (C) acetate, (D) glutamate, or (E) glutamate and acetate, each at 0.2%. (F–I) Growth curves of WCDC1551, Δ hoasCDC1551, Δ hoasCDC1551::hoas, hoas::tnCDC1551, and hoas::tnCDC1551::hoas in modified Sauton’s minimal medium supplemented with either (F) glycerol, (G) acetate, (E) glutamate, or (I) glutamate and acetate, each at 0.2%. All results are representative of at least three experiments.