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. 2015 Oct 15;6:8640. doi: 10.1038/ncomms9640

Figure 2. miR-582-3p is overexpressed in NSCLC cell lines and tissues.

Figure 2

(a) Expression of miR-582-3p and miR-582-5p in human lung cancer clinical specimens of human lung cancer using the TCGA miRNA HiSeq expression array data. (b) RT–qPCR analysis of miR-582-3p expression in NLEC normal lung epithelial cells, including primary NLECs, the immortalized human bronchial epithelial cell line BEAS-2B, and a panel of eight human NSCLC cell lines. (c) Relative expression of miR-582-3p in 8 pairs of NSCLC tumour tissues (T) and their corresponding adjacent non-cancerous tissues (ANT). (d) miR-582-3p is upregulated in all 8 NSCLC tumor specimens compared with paired adjacent non-tumour tissue based on ISH. Scale bar, 50 μm. (e) Northern blotblotting analysis of miR-582-3p in the indicated tissues and cell lines. U6 was used as an internal reference. (f) Absolute quantification of miR-582-3p expression in the indicated tissue specimens and cell lines using real-time PCR and a standard curve.. Each bar represents the mean±s.e.m. derived from three independent experiments. A two-tailed Student's t-test was used for statistical analysis (*P<0.05).