Figure 2.

Akt and GSK3 phosphorylation are not modified in WIP ^−/− neurons. Soluble extracts were obtained from murine primary cortical neurons from WT or WIP ^−/− embryos and maintained in suspension (0) or cultured at high density for 1, 2, 3, 24, or 48 h on PLL‐coated plates. Proteins analyzed in WB (25 μg/lane) were probed with antibodies to phosphoepitopes (A,B) Akt‐pThr³⁰⁸, (C,D) Akt‐pSer⁴⁷³ or (E,F) GSK3‐pSer^21/9. Equivalent protein loading was confirmed using anti‐Akt or ‐GSK3 antibodies. Images show one representative experiment. Data show as mean ± SEM; from four independent experiments; ns, not significant (two‐way ANOVA analysis).