Figure 6.

Subcellular S6K‐pThr³⁸⁹ distribution is modified in WIP ^−/− neurons. Representative images of hippocampal neurons from WT and WIP ^−/− murine embryos seeded on PLL‐coated coverslips (24 h), fixed and stained with TRITC‐phalloidin (F‐actin, red) and Alexa488‐anti‐S6K‐pThr³⁸⁹ (green) (A) or Alexa488‐anti‐S6K (green) (C). Scale bar, 10 μm for both. Quantification of integrated fluorescence intensity of S6K‐pThr³⁸⁹ (B) and anti‐S6K (D) for control and WIP ^−/− neurons. (E) Representative images of neuron growth cones obtained following the experimental design described for Fig. 5A and C. Scale bar, 5 μm. (F) Quantification of the spatial overlap of Alexa488‐anti‐S6K‐pThr³⁸⁹ signal on TRITC‐phalloidin signal in actin‐sparse or ‐rich (dotted outline) areas. n = 12 growth cones per genotype, from two independent experiments. Student's t‐test; **P < 0.01. (G) Representative images of neuritic F‐actin patches acquired following the experimental design described for Fig. 5A and C. Scale bar, 1 μm. (H) Quantification of the spatial overlap of Alexa488‐anti‐S6K‐pThr³⁸⁹ signal on TRITC‐phalloidin signal in actin‐sparse (light blue dotted outline) or ‐rich (white dotted outline) areas. n = 60 F‐actin patches or average of either side of them per genotype, from two independent experiments. *P < 0.05; ***P < 0.001 (Student's t‐test).